diff --git a/beacon-chain/cache/subnet_ids.go b/beacon-chain/cache/subnet_ids.go
index e47bf8288f..20273ade01 100644
--- a/beacon-chain/cache/subnet_ids.go
+++ b/beacon-chain/cache/subnet_ids.go
@@ -24,6 +24,8 @@ type subnetIDs struct {
 // SubnetIDs for attester and aggregator.
 var SubnetIDs = newSubnetIDs()
 
+var subnetKey = "persistent-subnets"
+
 func newSubnetIDs() *subnetIDs {
 	// Given a node can calculate committee assignments of current epoch and next epoch.
 	// Max size is set to 2 epoch length.
@@ -91,11 +93,11 @@ func (s *subnetIDs) GetAggregatorSubnetIDs(slot primitives.Slot) []uint64 {
 
 // GetPersistentSubnets retrieves the persistent subnet and expiration time of that validator's
 // subscription.
-func (s *subnetIDs) GetPersistentSubnets(pubkey []byte) ([]uint64, bool, time.Time) {
+func (s *subnetIDs) GetPersistentSubnets() ([]uint64, bool, time.Time) {
 	s.subnetsLock.RLock()
 	defer s.subnetsLock.RUnlock()
 
-	id, duration, ok := s.persistentSubnets.GetWithExpiration(string(pubkey))
+	id, duration, ok := s.persistentSubnets.GetWithExpiration(subnetKey)
 	if !ok {
 		return []uint64{}, ok, time.Time{}
 	}
@@ -122,11 +124,11 @@ func (s *subnetIDs) GetAllSubnets() []uint64 {
 
 // AddPersistentCommittee adds the relevant committee for that particular validator along with its
 // expiration period.
-func (s *subnetIDs) AddPersistentCommittee(pubkey []byte, comIndex []uint64, duration time.Duration) {
+func (s *subnetIDs) AddPersistentCommittee(comIndex []uint64, duration time.Duration) {
 	s.subnetsLock.Lock()
 	defer s.subnetsLock.Unlock()
 
-	s.persistentSubnets.Set(string(pubkey), comIndex, duration)
+	s.persistentSubnets.Set(subnetKey, comIndex, duration)
 }
 
 // EmptyAllCaches empties out all the related caches and flushes any stored
diff --git a/beacon-chain/cache/subnet_ids_test.go b/beacon-chain/cache/subnet_ids_test.go
index c94d8ffa52..67652c3d81 100644
--- a/beacon-chain/cache/subnet_ids_test.go
+++ b/beacon-chain/cache/subnet_ids_test.go
@@ -3,10 +3,8 @@ package cache
 import (
 	"testing"
 
-	fieldparams "github.com/prysmaticlabs/prysm/v4/config/fieldparams"
 	"github.com/prysmaticlabs/prysm/v4/consensus-types/primitives"
 	"github.com/prysmaticlabs/prysm/v4/testing/assert"
-	"github.com/prysmaticlabs/prysm/v4/testing/require"
 )
 
 func TestSubnetIDsCache_RoundTrip(t *testing.T) {
@@ -46,21 +44,8 @@ func TestSubnetIDsCache_RoundTrip(t *testing.T) {
 func TestSubnetIDsCache_PersistentCommitteeRoundtrip(t *testing.T) {
 	c := newSubnetIDs()
 
-	for i := 0; i < 20; i++ {
-		pubkey := [fieldparams.BLSPubkeyLength]byte{byte(i)}
-		c.AddPersistentCommittee(pubkey[:], []uint64{uint64(i)}, 0)
-	}
+	c.AddPersistentCommittee([]uint64{0, 1, 2, 7, 8}, 0)
 
-	for i := uint64(0); i < 20; i++ {
-		pubkey := [fieldparams.BLSPubkeyLength]byte{byte(i)}
-
-		idxs, ok, _ := c.GetPersistentSubnets(pubkey[:])
-		if !ok {
-			t.Errorf("Couldn't find entry in cache for pubkey %#x", pubkey)
-			continue
-		}
-		require.Equal(t, i, idxs[0])
-	}
 	coms := c.GetAllSubnets()
-	assert.Equal(t, 20, len(coms))
+	assert.Equal(t, 5, len(coms))
 }
diff --git a/beacon-chain/p2p/BUILD.bazel b/beacon-chain/p2p/BUILD.bazel
index 6ed282db9e..2a0e6ea45e 100644
--- a/beacon-chain/p2p/BUILD.bazel
+++ b/beacon-chain/p2p/BUILD.bazel
@@ -77,6 +77,7 @@ go_library(
         "@com_github_ethereum_go_ethereum//p2p/discover:go_default_library",
         "@com_github_ethereum_go_ethereum//p2p/enode:go_default_library",
         "@com_github_ethereum_go_ethereum//p2p/enr:go_default_library",
+        "@com_github_holiman_uint256//:go_default_library",
         "@com_github_kr_pretty//:go_default_library",
         "@com_github_libp2p_go_libp2p//:go_default_library",
         "@com_github_libp2p_go_libp2p//config:go_default_library",
@@ -164,6 +165,7 @@ go_test(
         "//testing/require:go_default_library",
         "//testing/util:go_default_library",
         "//time:go_default_library",
+        "//time/slots:go_default_library",
         "@com_github_ethereum_go_ethereum//crypto:go_default_library",
         "@com_github_ethereum_go_ethereum//p2p/discover:go_default_library",
         "@com_github_ethereum_go_ethereum//p2p/enode:go_default_library",
diff --git a/beacon-chain/p2p/discovery.go b/beacon-chain/p2p/discovery.go
index a56806e438..fa05250567 100644
--- a/beacon-chain/p2p/discovery.go
+++ b/beacon-chain/p2p/discovery.go
@@ -42,6 +42,12 @@ func (s *Service) RefreshENR() {
 	if s.dv5Listener == nil || !s.isInitialized() {
 		return
 	}
+	currEpoch := slots.ToEpoch(slots.CurrentSlot(uint64(s.genesisTime.Unix())))
+	if err := initializePersistentSubnets(s.dv5Listener.LocalNode().ID(), currEpoch); err != nil {
+		log.WithError(err).Error("Could not initialize persistent subnets")
+		return
+	}
+
 	bitV := bitfield.NewBitvector64()
 	committees := cache.SubnetIDs.GetAllSubnets()
 	for _, idx := range committees {
@@ -52,8 +58,8 @@ func (s *Service) RefreshENR() {
 		log.WithError(err).Error("Could not retrieve att bitfield")
 		return
 	}
+
 	// Compare current epoch with our fork epochs
-	currEpoch := slots.ToEpoch(slots.CurrentSlot(uint64(s.genesisTime.Unix())))
 	altairForkEpoch := params.BeaconConfig().AltairForkEpoch
 	switch {
 	// Altair Behaviour
diff --git a/beacon-chain/p2p/discovery_test.go b/beacon-chain/p2p/discovery_test.go
index 9a2701884b..eafa409cf7 100644
--- a/beacon-chain/p2p/discovery_test.go
+++ b/beacon-chain/p2p/discovery_test.go
@@ -36,6 +36,7 @@ import (
 	"github.com/prysmaticlabs/prysm/v4/runtime/version"
 	"github.com/prysmaticlabs/prysm/v4/testing/assert"
 	"github.com/prysmaticlabs/prysm/v4/testing/require"
+	"github.com/prysmaticlabs/prysm/v4/time/slots"
 	logTest "github.com/sirupsen/logrus/hooks/test"
 )
 
@@ -364,7 +365,15 @@ func TestRefreshENR_ForkBoundaries(t *testing.T) {
 				return s
 			},
 			postValidation: func(t *testing.T, s *Service) {
-				assert.DeepEqual(t, bitfield.NewBitvector64(), s.metaData.AttnetsBitfield())
+				currEpoch := slots.ToEpoch(slots.CurrentSlot(uint64(s.genesisTime.Unix())))
+				subs, err := computeSubscribedSubnets(s.dv5Listener.LocalNode().ID(), currEpoch)
+				assert.NoError(t, err)
+
+				bitV := bitfield.NewBitvector64()
+				for _, idx := range subs {
+					bitV.SetBitAt(idx, true)
+				}
+				assert.DeepEqual(t, bitV, s.metaData.AttnetsBitfield())
 			},
 		},
 		{
@@ -382,7 +391,7 @@ func TestRefreshENR_ForkBoundaries(t *testing.T) {
 				s.dv5Listener = listener
 				s.metaData = wrapper.WrappedMetadataV0(new(ethpb.MetaDataV0))
 				s.updateSubnetRecordWithMetadata([]byte{0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x01})
-				cache.SubnetIDs.AddPersistentCommittee([]byte{'A'}, []uint64{1, 2, 3, 23}, 0)
+				cache.SubnetIDs.AddPersistentCommittee([]uint64{1, 2, 3, 23}, 0)
 				return s
 			},
 			postValidation: func(t *testing.T, s *Service) {
@@ -411,7 +420,7 @@ func TestRefreshENR_ForkBoundaries(t *testing.T) {
 				s.dv5Listener = listener
 				s.metaData = wrapper.WrappedMetadataV0(new(ethpb.MetaDataV0))
 				s.updateSubnetRecordWithMetadata([]byte{0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x01})
-				cache.SubnetIDs.AddPersistentCommittee([]byte{'A'}, []uint64{1, 2, 3, 23}, 0)
+				cache.SubnetIDs.AddPersistentCommittee([]uint64{1, 2, 3, 23}, 0)
 				return s
 			},
 			postValidation: func(t *testing.T, s *Service) {
@@ -447,7 +456,15 @@ func TestRefreshENR_ForkBoundaries(t *testing.T) {
 			postValidation: func(t *testing.T, s *Service) {
 				assert.Equal(t, version.Altair, s.metaData.Version())
 				assert.DeepEqual(t, bitfield.Bitvector4{0x00}, s.metaData.MetadataObjV1().Syncnets)
-				assert.DeepEqual(t, bitfield.Bitvector64{0x0, 0x0, 0x0, 0x0, 0x0, 0x0, 0x0, 0x0}, s.metaData.AttnetsBitfield())
+				currEpoch := slots.ToEpoch(slots.CurrentSlot(uint64(s.genesisTime.Unix())))
+				subs, err := computeSubscribedSubnets(s.dv5Listener.LocalNode().ID(), currEpoch)
+				assert.NoError(t, err)
+
+				bitV := bitfield.NewBitvector64()
+				for _, idx := range subs {
+					bitV.SetBitAt(idx, true)
+				}
+				assert.DeepEqual(t, bitV, s.metaData.AttnetsBitfield())
 			},
 		},
 		{
@@ -472,7 +489,7 @@ func TestRefreshENR_ForkBoundaries(t *testing.T) {
 				s.dv5Listener = listener
 				s.metaData = wrapper.WrappedMetadataV0(new(ethpb.MetaDataV0))
 				s.updateSubnetRecordWithMetadata([]byte{0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00})
-				cache.SubnetIDs.AddPersistentCommittee([]byte{'A'}, []uint64{1, 2, 3, 23}, 0)
+				cache.SubnetIDs.AddPersistentCommittee([]uint64{1, 2, 3, 23}, 0)
 				cache.SyncSubnetIDs.AddSyncCommitteeSubnets([]byte{'A'}, 0, []uint64{0, 1}, 0)
 				return s
 			},
diff --git a/beacon-chain/p2p/subnets.go b/beacon-chain/p2p/subnets.go
index 1cba687dd4..66b9252b95 100644
--- a/beacon-chain/p2p/subnets.go
+++ b/beacon-chain/p2p/subnets.go
@@ -4,13 +4,20 @@ import (
 	"context"
 	"strings"
 	"sync"
+	"time"
 
 	"github.com/ethereum/go-ethereum/p2p/enode"
 	"github.com/ethereum/go-ethereum/p2p/enr"
+	"github.com/holiman/uint256"
 	"github.com/pkg/errors"
 	"github.com/prysmaticlabs/go-bitfield"
+	"github.com/prysmaticlabs/prysm/v4/beacon-chain/cache"
+	"github.com/prysmaticlabs/prysm/v4/beacon-chain/core/helpers"
 	"github.com/prysmaticlabs/prysm/v4/cmd/beacon-chain/flags"
+	"github.com/prysmaticlabs/prysm/v4/consensus-types/primitives"
 	"github.com/prysmaticlabs/prysm/v4/consensus-types/wrapper"
+	"github.com/prysmaticlabs/prysm/v4/crypto/hash"
+	"github.com/prysmaticlabs/prysm/v4/encoding/bytesutil"
 	mathutil "github.com/prysmaticlabs/prysm/v4/math"
 	"go.opencensus.io/trace"
 
@@ -178,6 +185,82 @@ func (s *Service) updateSubnetRecordWithMetadataV2(bitVAtt bitfield.Bitvector64,
 	})
 }
 
+func initializePersistentSubnets(id enode.ID, epoch primitives.Epoch) error {
+	_, ok, expTime := cache.SubnetIDs.GetPersistentSubnets()
+	if ok && expTime.After(time.Now()) {
+		return nil
+	}
+	subs, err := computeSubscribedSubnets(id, epoch)
+	if err != nil {
+		return err
+	}
+	newExpTime := computeSubscriptionExpirationTime(id, epoch)
+	cache.SubnetIDs.AddPersistentCommittee(subs, newExpTime)
+	return nil
+}
+
+// Spec pseudocode definition:
+//
+// def compute_subscribed_subnets(node_id: NodeID, epoch: Epoch) -> Sequence[SubnetID]:
+//
+//	return [compute_subscribed_subnet(node_id, epoch, index) for index in range(SUBNETS_PER_NODE)]
+func computeSubscribedSubnets(nodeID enode.ID, epoch primitives.Epoch) ([]uint64, error) {
+	subs := []uint64{}
+	for i := uint64(0); i < params.BeaconConfig().SubnetsPerNode; i++ {
+		sub, err := computeSubscribedSubnet(nodeID, epoch, i)
+		if err != nil {
+			return nil, err
+		}
+		subs = append(subs, sub)
+	}
+	return subs, nil
+}
+
+//	Spec pseudocode definition:
+//
+// def compute_subscribed_subnet(node_id: NodeID, epoch: Epoch, index: int) -> SubnetID:
+//
+//	node_id_prefix = node_id >> (NODE_ID_BITS - ATTESTATION_SUBNET_PREFIX_BITS)
+//	node_offset = node_id % EPOCHS_PER_SUBNET_SUBSCRIPTION
+//	permutation_seed = hash(uint_to_bytes(uint64((epoch + node_offset) // EPOCHS_PER_SUBNET_SUBSCRIPTION)))
+//	permutated_prefix = compute_shuffled_index(
+//	    node_id_prefix,
+//	    1 << ATTESTATION_SUBNET_PREFIX_BITS,
+//	    permutation_seed,
+//	)
+//	return SubnetID((permutated_prefix + index) % ATTESTATION_SUBNET_COUNT)
+func computeSubscribedSubnet(nodeID enode.ID, epoch primitives.Epoch, index uint64) (uint64, error) {
+	nodeOffset, nodeIdPrefix := computeOffsetAndPrefix(nodeID)
+	seedInput := (nodeOffset + uint64(epoch)) / params.BeaconConfig().EpochsPerSubnetSubscription
+	permSeed := hash.Hash(bytesutil.Bytes8(seedInput))
+	permutatedPrefix, err := helpers.ComputeShuffledIndex(primitives.ValidatorIndex(nodeIdPrefix), 1<<params.BeaconConfig().AttestationSubnetPrefixBits, permSeed, true)
+	if err != nil {
+		return 0, err
+	}
+	subnet := (uint64(permutatedPrefix) + index) % params.BeaconNetworkConfig().AttestationSubnetCount
+	return subnet, nil
+}
+
+func computeSubscriptionExpirationTime(nodeID enode.ID, epoch primitives.Epoch) time.Duration {
+	nodeOffset, _ := computeOffsetAndPrefix(nodeID)
+	pastEpochs := (nodeOffset + uint64(epoch)) % params.BeaconConfig().EpochsPerSubnetSubscription
+	remEpochs := params.BeaconConfig().EpochsPerSubnetSubscription - pastEpochs
+	epochDuration := time.Duration(params.BeaconConfig().SlotsPerEpoch.Mul(params.BeaconConfig().SecondsPerSlot))
+	epochTime := time.Duration(remEpochs) * epochDuration
+	return epochTime * time.Second
+}
+
+func computeOffsetAndPrefix(nodeID enode.ID) (uint64, uint64) {
+	num := uint256.NewInt(0).SetBytes(nodeID.Bytes())
+	remBits := params.BeaconConfig().NodeIdBits - params.BeaconConfig().AttestationSubnetPrefixBits
+	// Number of bits left will be representable by a uint64 value.
+	nodeIdPrefix := num.Rsh(num, uint(remBits)).Uint64()
+	// Reinitialize big int.
+	num = uint256.NewInt(0).SetBytes(nodeID.Bytes())
+	nodeOffset := num.Mod(num, uint256.NewInt(params.BeaconConfig().EpochsPerSubnetSubscription)).Uint64()
+	return nodeOffset, nodeIdPrefix
+}
+
 // Initializes a bitvector of attestation subnets beacon nodes is subscribed to
 // and creates a new ENR entry with its default value.
 func initializeAttSubnets(node *enode.LocalNode) *enode.LocalNode {
diff --git a/beacon-chain/p2p/subnets_test.go b/beacon-chain/p2p/subnets_test.go
index b7d2362698..b775c75d8b 100644
--- a/beacon-chain/p2p/subnets_test.go
+++ b/beacon-chain/p2p/subnets_test.go
@@ -488,3 +488,38 @@ func Test_SyncSubnets(t *testing.T) {
 		})
 	}
 }
+
+func TestSubnetComputation(t *testing.T) {
+	db, err := enode.OpenDB("")
+	assert.NoError(t, err)
+	defer db.Close()
+	priv, _, err := crypto.GenerateSecp256k1Key(rand.Reader)
+	assert.NoError(t, err)
+	convertedKey, err := ecdsaprysm.ConvertFromInterfacePrivKey(priv)
+	assert.NoError(t, err)
+	localNode := enode.NewLocalNode(db, convertedKey)
+
+	retrievedSubnets, err := computeSubscribedSubnets(localNode.ID(), 1000)
+	assert.NoError(t, err)
+	assert.Equal(t, retrievedSubnets[0]+1, retrievedSubnets[1])
+}
+
+func TestInitializePersistentSubnets(t *testing.T) {
+	cache.SubnetIDs.EmptyAllCaches()
+	defer cache.SubnetIDs.EmptyAllCaches()
+
+	db, err := enode.OpenDB("")
+	assert.NoError(t, err)
+	defer db.Close()
+	priv, _, err := crypto.GenerateSecp256k1Key(rand.Reader)
+	assert.NoError(t, err)
+	convertedKey, err := ecdsaprysm.ConvertFromInterfacePrivKey(priv)
+	assert.NoError(t, err)
+	localNode := enode.NewLocalNode(db, convertedKey)
+
+	assert.NoError(t, initializePersistentSubnets(localNode.ID(), 10000))
+	subs, ok, expTime := cache.SubnetIDs.GetPersistentSubnets()
+	assert.Equal(t, true, ok)
+	assert.Equal(t, 2, len(subs))
+	assert.Equal(t, true, expTime.After(time.Now()))
+}
diff --git a/beacon-chain/rpc/core/BUILD.bazel b/beacon-chain/rpc/core/BUILD.bazel
index 87a23a8d35..eab9b31241 100644
--- a/beacon-chain/rpc/core/BUILD.bazel
+++ b/beacon-chain/rpc/core/BUILD.bazel
@@ -30,7 +30,6 @@ go_library(
         "//consensus-types/primitives:go_default_library",
         "//consensus-types/validator:go_default_library",
         "//crypto/bls:go_default_library",
-        "//crypto/rand:go_default_library",
         "//encoding/bytesutil:go_default_library",
         "//proto/prysm/v1alpha1:go_default_library",
         "//runtime/version:go_default_library",
diff --git a/beacon-chain/rpc/core/validator.go b/beacon-chain/rpc/core/validator.go
index b63090c085..dd766d6997 100644
--- a/beacon-chain/rpc/core/validator.go
+++ b/beacon-chain/rpc/core/validator.go
@@ -22,7 +22,6 @@ import (
 	"github.com/prysmaticlabs/prysm/v4/consensus-types/primitives"
 	"github.com/prysmaticlabs/prysm/v4/consensus-types/validator"
 	"github.com/prysmaticlabs/prysm/v4/crypto/bls"
-	"github.com/prysmaticlabs/prysm/v4/crypto/rand"
 	"github.com/prysmaticlabs/prysm/v4/encoding/bytesutil"
 	ethpb "github.com/prysmaticlabs/prysm/v4/proto/prysm/v1alpha1"
 	"github.com/prysmaticlabs/prysm/v4/runtime/version"
@@ -310,46 +309,6 @@ func (s *Service) AggregatedSigAndAggregationBits(
 	return aggregatedSig, bits, nil
 }
 
-// AssignValidatorToSubnet checks the status and pubkey of a particular validator
-// to discern whether persistent subnets need to be registered for them.
-func AssignValidatorToSubnet(pubkey []byte, status validator.Status) {
-	if status != validator.Active {
-		return
-	}
-	assignValidatorToSubnet(pubkey)
-}
-
-// AssignValidatorToSubnetProto checks the status and pubkey of a particular validator
-// to discern whether persistent subnets need to be registered for them.
-//
-// It has a Proto suffix because the status is a protobuf type.
-func AssignValidatorToSubnetProto(pubkey []byte, status ethpb.ValidatorStatus) {
-	if status != ethpb.ValidatorStatus_ACTIVE && status != ethpb.ValidatorStatus_EXITING {
-		return
-	}
-	assignValidatorToSubnet(pubkey)
-}
-
-func assignValidatorToSubnet(pubkey []byte) {
-	_, ok, expTime := cache.SubnetIDs.GetPersistentSubnets(pubkey)
-	if ok && expTime.After(prysmTime.Now()) {
-		return
-	}
-	epochDuration := time.Duration(params.BeaconConfig().SlotsPerEpoch.Mul(params.BeaconConfig().SecondsPerSlot))
-	var assignedIdxs []uint64
-	randGen := rand.NewGenerator()
-	for i := uint64(0); i < params.BeaconConfig().RandomSubnetsPerValidator; i++ {
-		assignedIdx := randGen.Intn(int(params.BeaconNetworkConfig().AttestationSubnetCount))
-		assignedIdxs = append(assignedIdxs, uint64(assignedIdx))
-	}
-
-	assignedDuration := uint64(randGen.Intn(int(params.BeaconConfig().EpochsPerRandomSubnetSubscription)))
-	assignedDuration += params.BeaconConfig().EpochsPerRandomSubnetSubscription
-
-	totalDuration := epochDuration * time.Duration(assignedDuration)
-	cache.SubnetIDs.AddPersistentCommittee(pubkey, assignedIdxs, totalDuration*time.Second)
-}
-
 // GetAttestationData requests that the beacon node produces attestation data for
 // the requested committee index and slot based on the nodes current head.
 func (s *Service) GetAttestationData(
diff --git a/beacon-chain/rpc/eth/config/handlers_test.go b/beacon-chain/rpc/eth/config/handlers_test.go
index 6848283c03..524786590a 100644
--- a/beacon-chain/rpc/eth/config/handlers_test.go
+++ b/beacon-chain/rpc/eth/config/handlers_test.go
@@ -172,7 +172,7 @@ func TestGetSpec(t *testing.T) {
 	data, ok := resp.Data.(map[string]interface{})
 	require.Equal(t, true, ok)
 
-	assert.Equal(t, 113, len(data))
+	assert.Equal(t, 118, len(data))
 	for k, v := range data {
 		switch k {
 		case "CONFIG_NAME":
@@ -418,6 +418,16 @@ func TestGetSpec(t *testing.T) {
 		case "MAX_REQUEST_LIGHT_CLIENT_UPDATES":
 			assert.Equal(t, "128", v)
 		case "SAFE_SLOTS_TO_IMPORT_OPTIMISTICALLY":
+		case "NODE_ID_BITS":
+			assert.Equal(t, "256", v)
+		case "ATTESTATION_SUBNET_EXTRA_BITS":
+			assert.Equal(t, "0", v)
+		case "ATTESTATION_SUBNET_PREFIX_BITS":
+			assert.Equal(t, "6", v)
+		case "SUBNETS_PER_NODE":
+			assert.Equal(t, "2", v)
+		case "EPOCHS_PER_SUBNET_SUBSCRIPTION":
+			assert.Equal(t, "256", v)
 		default:
 			t.Errorf("Incorrect key: %s", k)
 		}
diff --git a/beacon-chain/rpc/eth/validator/handlers.go b/beacon-chain/rpc/eth/validator/handlers.go
index cae2b9a750..96b4966f7f 100644
--- a/beacon-chain/rpc/eth/validator/handlers.go
+++ b/beacon-chain/rpc/eth/validator/handlers.go
@@ -381,15 +381,6 @@ func (s *Server) SubmitBeaconCommitteeSubscription(w http.ResponseWriter, r *htt
 			cache.SubnetIDs.AddAggregatorSubnetID(sub.Slot, subnet)
 		}
 	}
-	for _, val := range validators {
-		valStatus, err := rpchelpers.ValidatorStatus(val, currEpoch)
-		if err != nil {
-			http2.HandleError(w, "Could not retrieve validator status: "+err.Error(), http.StatusInternalServerError)
-			return
-		}
-		pubkey := val.PublicKey()
-		core.AssignValidatorToSubnet(pubkey[:], valStatus)
-	}
 }
 
 // GetAttestationData requests that the beacon node produces attestation data for
diff --git a/beacon-chain/rpc/eth/validator/handlers_test.go b/beacon-chain/rpc/eth/validator/handlers_test.go
index 96155f7c97..7e81ce5c94 100644
--- a/beacon-chain/rpc/eth/validator/handlers_test.go
+++ b/beacon-chain/rpc/eth/validator/handlers_test.go
@@ -737,25 +737,6 @@ func TestSubmitBeaconCommitteeSubscription(t *testing.T) {
 		require.Equal(t, 1, len(subnets))
 		assert.Equal(t, uint64(5), subnets[0])
 	})
-	t.Run("validators assigned to subnets", func(t *testing.T) {
-		cache.SubnetIDs.EmptyAllCaches()
-
-		var body bytes.Buffer
-		_, err := body.WriteString(multipleBeaconCommitteeContribution2)
-		require.NoError(t, err)
-		request := httptest.NewRequest(http.MethodPost, "http://example.com", &body)
-		writer := httptest.NewRecorder()
-		writer.Body = &bytes.Buffer{}
-
-		s.SubmitBeaconCommitteeSubscription(writer, request)
-		assert.Equal(t, http.StatusOK, writer.Code)
-		subnets, ok, _ := cache.SubnetIDs.GetPersistentSubnets(pubkeys[1])
-		require.Equal(t, true, ok, "subnet for validator 1 not found")
-		assert.Equal(t, 1, len(subnets))
-		subnets, ok, _ = cache.SubnetIDs.GetPersistentSubnets(pubkeys[2])
-		require.Equal(t, true, ok, "subnet for validator 2 not found")
-		assert.Equal(t, 1, len(subnets))
-	})
 	t.Run("no body", func(t *testing.T) {
 		request := httptest.NewRequest(http.MethodPost, "http://example.com", nil)
 		writer := httptest.NewRecorder()
diff --git a/beacon-chain/rpc/prysm/v1alpha1/validator/assignments.go b/beacon-chain/rpc/prysm/v1alpha1/validator/assignments.go
index 835ea3ea53..2299f7811b 100644
--- a/beacon-chain/rpc/prysm/v1alpha1/validator/assignments.go
+++ b/beacon-chain/rpc/prysm/v1alpha1/validator/assignments.go
@@ -227,9 +227,6 @@ func (vs *Server) duties(ctx context.Context, req *ethpb.DutiesRequest) (*ethpb.
 
 		validatorAssignments = append(validatorAssignments, assignment)
 		nextValidatorAssignments = append(nextValidatorAssignments, nextAssignment)
-		// Assign relevant validator to subnet.
-		core.AssignValidatorToSubnetProto(pubKey, assignment.Status)
-		core.AssignValidatorToSubnetProto(pubKey, nextAssignment.Status)
 	}
 	// Prune payload ID cache for any slots before request slot.
 	vs.ProposerSlotIndexCache.PrunePayloadIDs(epochStartSlot)
@@ -244,6 +241,5 @@ func (vs *Server) duties(ctx context.Context, req *ethpb.DutiesRequest) (*ethpb.
 // AssignValidatorToSubnet checks the status and pubkey of a particular validator
 // to discern whether persistent subnets need to be registered for them.
 func (vs *Server) AssignValidatorToSubnet(_ context.Context, req *ethpb.AssignValidatorToSubnetRequest) (*emptypb.Empty, error) {
-	core.AssignValidatorToSubnetProto(req.PublicKey, req.Status)
 	return &emptypb.Empty{}, nil
 }
diff --git a/beacon-chain/rpc/prysm/v1alpha1/validator/assignments_test.go b/beacon-chain/rpc/prysm/v1alpha1/validator/assignments_test.go
index 7de63141ae..d8ccd801c3 100644
--- a/beacon-chain/rpc/prysm/v1alpha1/validator/assignments_test.go
+++ b/beacon-chain/rpc/prysm/v1alpha1/validator/assignments_test.go
@@ -17,7 +17,6 @@ import (
 	"github.com/prysmaticlabs/prysm/v4/beacon-chain/core/helpers"
 	"github.com/prysmaticlabs/prysm/v4/beacon-chain/core/transition"
 	mockExecution "github.com/prysmaticlabs/prysm/v4/beacon-chain/execution/testing"
-	"github.com/prysmaticlabs/prysm/v4/beacon-chain/rpc/core"
 	mockSync "github.com/prysmaticlabs/prysm/v4/beacon-chain/sync/initial-sync/testing"
 	fieldparams "github.com/prysmaticlabs/prysm/v4/config/fieldparams"
 	"github.com/prysmaticlabs/prysm/v4/config/params"
@@ -604,21 +603,6 @@ func TestStreamDuties_OK_ChainReorg(t *testing.T) {
 	cancel()
 }
 
-func TestAssignValidatorToSubnet(t *testing.T) {
-	k := pubKey(3)
-
-	core.AssignValidatorToSubnetProto(k, ethpb.ValidatorStatus_ACTIVE)
-	coms, ok, exp := cache.SubnetIDs.GetPersistentSubnets(k)
-	require.Equal(t, true, ok, "No cache entry found for validator")
-	assert.Equal(t, params.BeaconConfig().RandomSubnetsPerValidator, uint64(len(coms)))
-	epochDuration := time.Duration(params.BeaconConfig().SlotsPerEpoch.Mul(params.BeaconConfig().SecondsPerSlot))
-	totalTime := time.Duration(params.BeaconConfig().EpochsPerRandomSubnetSubscription) * epochDuration * time.Second
-	receivedTime := time.Until(exp.Round(time.Second))
-	if receivedTime < totalTime {
-		t.Fatalf("Expiration time of %f was less than expected duration of %f ", receivedTime.Seconds(), totalTime.Seconds())
-	}
-}
-
 func BenchmarkCommitteeAssignment(b *testing.B) {
 
 	genesis := util.NewBeaconBlock()
diff --git a/config/params/config.go b/config/params/config.go
index 4991a25818..c7a27393fe 100644
--- a/config/params/config.go
+++ b/config/params/config.go
@@ -225,6 +225,13 @@ type BeaconChainConfig struct {
 
 	// Values introduced in Deneb hard fork
 	MaxPerEpochActivationChurnLimit uint64 `yaml:"MAX_PER_EPOCH_ACTIVATION_CHURN_LIMIT" spec:"true"` // MaxPerEpochActivationChurnLimit is the maximum amount of churn allotted for validator activation.
+
+	// Values related to the new subnet backbone
+	EpochsPerSubnetSubscription uint64 `yaml:"EPOCHS_PER_SUBNET_SUBSCRIPTION" spec:"true"` // EpochsPerSubnetSubscription specifies the minimum duration a validator is connected to their subnet.
+	AttestationSubnetExtraBits  uint64 `yaml:"ATTESTATION_SUBNET_EXTRA_BITS" spec:"true"`  // AttestationSubnetExtraBits is the number of extra bits of a NodeId to use when mapping to a subscribed subnet.
+	AttestationSubnetPrefixBits uint64 `yaml:"ATTESTATION_SUBNET_PREFIX_BITS" spec:"true"` // AttestationSubnetPrefixBits is defined as (ceillog2(ATTESTATION_SUBNET_COUNT) + ATTESTATION_SUBNET_EXTRA_BITS).
+	SubnetsPerNode              uint64 `yaml:"SUBNETS_PER_NODE" spec:"true"`               // SubnetsPerNode is the number of long-lived subnets a beacon node should be subscribed to.
+	NodeIdBits                  uint64 `yaml:"NODE_ID_BITS" spec:"true"`                   // NodeIdBits defines the bit length of a node id.
 }
 
 // InitializeForkSchedule initializes the schedules forks baked into the config.
diff --git a/config/params/loader.go b/config/params/loader.go
index 653609a90d..a42bbfaf22 100644
--- a/config/params/loader.go
+++ b/config/params/loader.go
@@ -209,6 +209,11 @@ func ConfigToYaml(cfg *BeaconChainConfig) []byte {
 		fmt.Sprintf("DEPOSIT_CONTRACT_ADDRESS: %s", cfg.DepositContractAddress),
 		fmt.Sprintf("DENEB_FORK_EPOCH: %d", cfg.DenebForkEpoch),
 		fmt.Sprintf("DENEB_FORK_VERSION: %#x", cfg.DenebForkVersion),
+		fmt.Sprintf("EPOCHS_PER_SUBNET_SUBSCRIPTION: %d", cfg.EpochsPerSubnetSubscription),
+		fmt.Sprintf("ATTESTATION_SUBNET_EXTRA_BITS: %d", cfg.AttestationSubnetExtraBits),
+		fmt.Sprintf("ATTESTATION_SUBNET_PREFIX_BITS: %d", cfg.AttestationSubnetPrefixBits),
+		fmt.Sprintf("SUBNETS_PER_NODE: %d", cfg.SubnetsPerNode),
+		fmt.Sprintf("NODE_ID_BITS: %d", cfg.NodeIdBits),
 	}
 
 	yamlFile := []byte(strings.Join(lines, "\n"))
diff --git a/config/params/loader_test.go b/config/params/loader_test.go
index 34b9694ddb..1318070465 100644
--- a/config/params/loader_test.go
+++ b/config/params/loader_test.go
@@ -25,13 +25,10 @@ import (
 var placeholderFields = []string{
 	"ATTESTATION_PROPAGATION_SLOT_RANGE",
 	"ATTESTATION_SUBNET_COUNT",
-	"ATTESTATION_SUBNET_EXTRA_BITS",
-	"ATTESTATION_SUBNET_PREFIX_BITS",
 	"EIP6110_FORK_EPOCH",
 	"EIP6110_FORK_VERSION",
 	"EIP7002_FORK_EPOCH",
 	"EIP7002_FORK_VERSION",
-	"EPOCHS_PER_SUBNET_SUBSCRIPTION",
 	"GOSSIP_MAX_SIZE",
 	"MAXIMUM_GOSSIP_CLOCK_DISPARITY",
 	"MAX_BLOBS_PER_BLOCK",
@@ -44,7 +41,6 @@ var placeholderFields = []string{
 	"MIN_EPOCHS_FOR_BLOB_SIDECARS_REQUESTS",
 	"MIN_EPOCHS_FOR_BLOCK_REQUESTS",
 	"RESP_TIMEOUT",
-	"SUBNETS_PER_NODE",
 	"TTFB_TIMEOUT",
 	"UPDATE_TIMEOUT",
 	"WHISK_EPOCHS_PER_SHUFFLING_PHASE",
diff --git a/config/params/mainnet_config.go b/config/params/mainnet_config.go
index f6f9e62380..42bf10cf96 100644
--- a/config/params/mainnet_config.go
+++ b/config/params/mainnet_config.go
@@ -274,6 +274,13 @@ var mainnetBeaconConfig = &BeaconChainConfig{
 	BlobsidecarSubnetCount: 6,
 
 	MaxPerEpochActivationChurnLimit: 8,
+
+	// Values related to the new subnet backbone
+	EpochsPerSubnetSubscription: 256,
+	AttestationSubnetExtraBits:  0,
+	AttestationSubnetPrefixBits: 6,
+	SubnetsPerNode:              2,
+	NodeIdBits:                  256,
 }
 
 // MainnetTestConfig provides a version of the mainnet config that has a different name
diff --git a/testing/endtoend/components/web3remotesigner.go b/testing/endtoend/components/web3remotesigner.go
index 4068b8f484..fe3c36357c 100644
--- a/testing/endtoend/components/web3remotesigner.go
+++ b/testing/endtoend/components/web3remotesigner.go
@@ -262,11 +262,7 @@ func createTestnetDir() (string, error) {
 	rawYaml = append(rawYaml, []byte(depContractStr)...)
 	rawYaml = append(rawYaml, params.NetworkConfigToYaml(params.BeaconNetworkConfig())...)
 
-	rawYaml = append(rawYaml, []byte("\nEPOCHS_PER_SUBNET_SUBSCRIPTION: 256\n")...)
 	rawYaml = append(rawYaml, []byte("\nMIN_EPOCHS_FOR_BLOCK_REQUESTS: 33024\n")...)
-	rawYaml = append(rawYaml, []byte("\nSUBNETS_PER_NODE: 2\n")...)
-	rawYaml = append(rawYaml, []byte("\nATTESTATION_SUBNET_EXTRA_BITS: 0\n")...)
-	rawYaml = append(rawYaml, []byte("\nATTESTATION_SUBNET_PREFIX_BITS: 6\n")...)
 
 	if err := file.MkdirAll(testNetDir); err != nil {
 		return "", err
diff --git a/validator/rpc/handlers_health_test.go b/validator/rpc/handlers_health_test.go
index 4e645e00e7..7be1938bc5 100644
--- a/validator/rpc/handlers_health_test.go
+++ b/validator/rpc/handlers_health_test.go
@@ -12,7 +12,7 @@ import (
 	"github.com/golang/mock/gomock"
 	"github.com/golang/protobuf/ptypes/empty"
 	"github.com/prysmaticlabs/prysm/v4/io/logs/mock"
-	"github.com/prysmaticlabs/prysm/v4/proto/prysm/v1alpha1"
+	eth "github.com/prysmaticlabs/prysm/v4/proto/prysm/v1alpha1"
 	pb "github.com/prysmaticlabs/prysm/v4/proto/prysm/v1alpha1"
 	"github.com/prysmaticlabs/prysm/v4/testing/require"
 	validatormock "github.com/prysmaticlabs/prysm/v4/testing/validator-mock"